|“||I am the queen of this forest, the mother of all witches!||”|
Witch Queen is a tall woman with a slim build. She has pink hair, which is pulled up under her hat, and teal eyes. Beneath both of her eyes are three dark blue teardrops. Her lips are the same shade of blue.
She wears a white, layered dress with each layer of the skirt having a large red eyespot. The bodice has a high neck and long sleeves. The skirt flares out into a large hoop.
She also wears a white conical hat with a wide brim. It has the same layered look as her dress but lacks the eyespots. Additionally the black feather hat band has a dark red flower in front and a collection of multicolored jewels on her left side.
From her left ear hangs a purple magic stone, and from her necklace hangs three black feathers.
Witch Queen distastes men and imperfection.
Within the Witches' Forest, Witch Queen imprisons Vanessa Enoteca as a child because she foresaw the potential of Vanessa's Thread Magic. Years later, Yami Sukehiro accidentally breaks into the cage after fighting with Witch Queen, and frees Vanessa, whom the queen allows to escape because she foresaw Vanessa's eventual return with a gift.
When Vanessa returns to the forest's gate and demands entrance, Witch Queen sends a flock of birds to grab the witch and pull her in. In her throne room, Witch Queen chastises Vanessa for failing to strengthen her magic and for being used by a man. In her anger, Witch Queen releases her mana and blasts a hole in the wall, but Vanessa is undeterred and demands to be taught how to dispel ancient curses. In return, Vanessa promises to remain as a slave in the forest.
Witch Queen has beaten Vanessa and is stepping on her when the intruders crash into the throne room. Asta demands to know what the queen is doing and for her to stop. However, the queen rebuffs him for intruding on her family affairs. He charges at her and Finral Roulacase warps him behind the queen, but she catches and holds Asta with a flock of birds. She notices Asta does not have any magic and realizes he is the one Vanessa wants to heal. She then reacts to the two armies that appeared on the outskirts of the forest.
After Ladros absorbs Salamander and shoots and knocks out Asta, Witch Queen revives the Black Bull and demands that he show who and what he is. She is impressed when he remains conscious as Anti Magic overflows from him. She also realizes that his ability to wield Anti Magic weapons comes not from being a descendant of the previous wielder but from his inability to hold mana. As Asta tears through Ladros' attacks, Witch Queen tells him to kill for Anti Magic seeks out and pursues all magic.
After Asta defeats Ladros, he notices how injured his friends are and declares that he will carry them all, but Witch Queen tells that it will be unnecessary, and appears from a flock of birds. She compliments him on defeating the two armies, but when he asks for her to heal everybody, she refuses. Instead, she restrains them with Scaffold of Fresh Blood. Taking control of and healing Asta's body, she forces him to draw his Demon-Slayer Sword and to release the flow of Anti Magic, and then prepares to have him kill everyone else. She decides to kill Noelle Silva first, noting the irony of one of the Clover Kingdom's nobles dying to that sword, but Asta blocks his right hand with his left, which frustrates the queen. Noelle tells Asta that she will dispel the magic controlling him, but the queen mocks her for underestimating the spell. Vanessa pleads for the queen to release everyone in return for her obedience, but Witch Queen refuses and states that by killing all of them, she will make sure such things never happen again and that she foretold Vanessa's return with a gift, which the queen did not know would be a man with Anti Magic weapons.
Vanessa denies any familial connection with Witch Queen and, in her anger and desperation, summons the red string of fate in the form of a cat, which Witch Queen recognizes and decides to test it by having Asta kill Noelle. The cat leaps to Asta's shoulder but does not stop his swing, which beheads Noelle, and Witch Queen declares Vanessa's magic to be imperfect. The queen then notices that Asta has not swung yet, so she commands him again to attack, but the swing misses Noelle and hits the scaffold instead. She has Asta stab Noelle. The cat leaps to his shoulder again, and suddenly Noelle is no longer stabbed and Asta redirects the attack with his left hand, stabbing the ground next to Noelle. Witch Queen commands Asta to kill Finral, and again the attack lands but somehow misses. Flustered, she has Asta attack the cat, which unthreads its middle, allowing the sword to pass through, and touches Asta's forehead. Asta's swing continues until the hilt hits his own head, freeing him from the queen's control.
Recognizing the cat's true ability, Witch Queen takes control of Vanessa and summons a large scythe of blood. As she flies forward, the cat touches her forehead and all of the queen's spells fall apart. The queen admits defeat and allows Vanessa to go free.
Nero flies over, landing on the queen's shoulder, and taps the magic stone hanging from her left ear. Once back in her throneroom, she gives the Black Bulls the stone and tells them about it, the elves, the possible connection to the White Night Eye, and Asta's swords. Before they leave, the queen hands Mars a bottled version of Puppet Bloodflow to use on the king of Diamond Kingdom, and Asta a bottled version of Curse-Breaking Blood Cocoon.
- Healing Magic: Witch Queen uses this form of magic for recovery purposes. Her spells can dispel ancient curses, mend shattered bones, and reinforce limbs.
- Bird Control: Witch Queen can control a flock of black birds. She often uses them to grab or hold people. They are obedient and strong enough to serve as a makeshift chair.
- Immense Magic Power: Witch Queen has enough mana to cover the entire Witches' Forestand overwhelm Ladros' ability to absorb and store magic. By simply releasing her mana, she blasts a large hole in the wall of her throne room. She can use multiple high-level spells in rapid succession without showing any signs of fatigue, and cause Noelle Silva's body to freeze up in fear from the sheer volume of her power.
- Fortune-Telling 「占い Uranai」: Witch Queen can foresee the future, although it is not always accurate.
- Grimoire: Witch Queen possesses a three-leaf clover grimoire that contains various blood-based spells.
- "...Imperfect..." 「...不完全... "...Fukanzen..."」
- "I am the queen of this forest, the mother of all witches!" 「私はこの森の女王すべての魔女の母なる存在...! "Watashi wa kono mori no joō subete no majo no hahanaru sonzai...!"」